產品貨號 :  mlR2709

英文名稱 :  CD150/SLAM

中文名稱 :  信號淋巴細胞活化分子CD150抗體

別    名 :  CD150 antigen; Cdw150; SLAM / CD150; Estm51; Ipo 3; Ipo3; MGC151472; MGC151476; Signaling lymphocytic activation molecule; Signaling lymphocytic activation molecule family member 1; Signaling lymphocytic activation molecule precursor; Slaf1; SLAM family, member 1; Slamf; Slamf1; SLAMF1 protein.  

研究領域 :  免疫學  

抗體來源 :  Rabbit

克隆類型 :  Polyclonal

交叉反應 :   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,

產品應用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  70kDa

細胞定位 :  細胞膜

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human CD150:231-335/335 <Cytoplasmic>

亞    型 :  IgG

純化方法 :  affinity purified by Protein A

儲 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

產品介紹 :   SLAM, a novel glycoprotein of 37kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) or CDw150, belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and the expression levels of membrane bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells.

Function:

High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

Subunit:

nteracts (via cytoplasmic domain) with SH2D1A, and withPTPN11. Interacts with INPP5D/SHIP1. Binds to Measles virus Hprotein and acts as a receptor for this virus.

Subcellular Location:

Cell membrane; Single-pass type I membrane protein. Note=Present on the surface of B-cells and T-cells.

Tissue Specificity:

Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.

Post-translational modifications:

Phosphorylated by FYN.

Similarity:

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:

Q13291

Gene ID:

6504

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD150（SLAM）為70kD的表面糖蛋白屬于免疫球蛋白基因超家族。SLAM有直接誘導CD4陽性T細胞克隆與活化前T細胞的作用。CD150抗原還可以增強抗原特異性增生與通過T細胞攜帶的CD4抗原產生細胞因子，特別是對干擾素-γ具有極強的上調節作用。SLAM與自身配體具有極高親和性，由SLAM同源親和細胞間的相互作用能夠刺激T淋巴細胞產生自身因子。CD150抗原還是風疹病毒細胞受體并且通過白介素10下調節。