產品貨號 :  mlR16788

英文名稱 :  KMT1B

中文名稱 :  賴氨酸N甲基轉移酶1B抗體

別    名 :  FLJ23414; H3 K9 HMTase 2; H3-K9-HMTase 2; Histone H3 K9 methyltransferase 2; Histone H3-K9 methyltransferase 2; Histone lysine N methyltransferase H3 lysine 9 specific 2; Histone lysine N methyltransferase SUV39H2; Histone-lysine N-methyltransferase SUV39H2; KMT1B; Lysine N methyltransferase 1B; Lysine N-methyltransferase 1B; sSuppressor of variegation 3 9 homolog 2 (Drosophila); Su(var)3 9 Drosophila homolog of 2; Su(var)3 9 homolog 2; Su(var)3-9 homolog 2; Suppressor of variegation 3 9 homolog 2; Suppressor of variegation 3-9 homolog 2; Suv39h2; SUV92_HUMAN.  

產品類型 :  甲基化抗體

研究領域 :  細胞生物  信號轉導  表觀遺傳學  

抗體來源 :  Rabbit

克隆類型 :  Polyclonal

交叉反應 :  Human, Mouse, Horse, Rabbit,

產品應用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  47kDa

細胞定位 :  細胞核

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human KMT1B:311-410/410

亞    型 :  IgG

純化方法 :  affinity purified by Protein A

儲 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

產品介紹background:

Distinct modifications of histone tails, such as acetylation, phosphorylation and methylation, regulate nuclear processes by organizing chromatin into higher order structures. Higher order chromatin influences chromosome function and epigenetic gene regulation. SUV39H2 is a 410 amino acid protein that localizes to the centromere and contains one SET domain, one pre-SET domain, one post-SET domain and one chromo domain. Expressed at high levels in adult testis, SUV39H2 functions as a histone methyltransferase that trimethylates the Lys-9 residue of Histone H3, thereby playing an essential role in establishing constitutive heterochromatin at pericentric and telomere regions. SUV39H2 conveys its enzymatic activity via its multiple catalytic domains, which are necessary for both stable binding of SUV39H2 to chromatin and for SUV39H2 methyltransferase activity. Multiple isoforms of SUV39H2 exist due to alternative splicing events.

Function:

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.

Subcellular Location:

Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.

Similarity:

Belongs to the histone-lysine methyltransferase family.

Suvar3-9 subfamily.

Contains 1 chromo domain.

Contains 1 post-SET domain.

Contains 1 pre-SET domain.

Contains 1 SET domain.

SWISS:

Q9H5I1

Gene ID:

79723

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.