產品貨號 :  mlR20433

英文名稱 :  FLI1

中文名稱 :  尤文肉瘤FLI1蛋白抗體

別    名 :  ERGB transcription factor; Ewing Sarcoma breakpoint region 2; EWSR 2; EWSR2; FLI 1; FLI 1 proto oncogene; FLI1; FLI1 EWS fusion gene; FLI1 proto oncogene; FLI1_HUMAN; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC 1; SIC1; Transcription factor ERGB; Viral integration region FLI1.  

研究領域 :  腫瘤  細胞生物  免疫學  染色質和核信號  細胞凋亡  細胞周期蛋白  表觀遺傳學  

抗體來源 :  Rabbit

克隆類型 :  Polyclonal

交叉反應 :   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,

產品應用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  50kDa

細胞定位 :  細胞核

性    狀 :  Lyophilized or Liquid

濃    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human FLI1:1-100/452

亞    型 :  IgG

純化方法 :  affinity purified by Protein A

儲 存 液 :  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存條件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

產品介紹background:

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Function:

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5-C[CA]GGAAGT-3

Subunit:

Can form homodimers or heterodimers with ETV6/TEL1.

Subcellular Location:

Nucleus.

DISEASE:

Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.

Similarity:

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Contains 1 PNT (pointed) domain.

SWISS:

Q01543

Gene ID:

2313

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品圖片